Coding

Part:BBa_K209411:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-09-30)

AarI C-D part, Lamellipodin (LPD) (775-1250)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 365
    Illegal PstI site found at 740
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 365
    Illegal PstI site found at 740
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 365
    Illegal PstI site found at 740
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 365
    Illegal PstI site found at 740
    Illegal AgeI site found at 246
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 710
    Illegal BsaI.rc site found at 657
    Illegal BsaI.rc site found at 724
    Illegal BsaI.rc site found at 1397


Design Notes

Cloned into pDONR221.

Source

References